List of Commonly Requested Auxotrophic Strains

Here is a list of auxotrophic strains organized by amino acid. The JW strains are all from the Keio collection of single gene knockouts and are therefore all in the same genetic background (strain BW25113) When another strain is listed, it is the strain that was most commonly requested/sent for that particular auxotrophy.

This is by no means an exhaustive list, and there are, in most cases, other genes in the biosynthetic pathway that can lead to auxotrophy.