Mutation asnB723(del)::kan
- Name: asnB723(del)::kan
- Type: Insertion
- Mutation of Gene: asnB
- Approx. Map Location: 15.02
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGATTCCGGGGATCCGTCGACC and ACAAGCAAACACAACAAGCAACAAATACCAGGTTAACGGAGAAGGTTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0660-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔasnB723::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514