Mutation fhuF774(del)::kan
- Name: fhuF774(del)::kan
- Type: Insertion
- Mutation of Gene: fhuF
- Approx. Map Location: 99.21
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ATGCCGGGTTTACTTCCCCGGCAGTGCTTTCATTTCAGCGTACAATCGCCATTCCGGGGATCCGTCGACC and TATCGCTATGATTAGCAATCATTATCATTTAGATTACTATCCCGATTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4331-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514, ΔfhuF774::kan