Mutation hflK724(del)::kan
- Name: hflK724(del)::kan
- Type: Insertion
- Mutation of Gene: hflK
- Approx. Map Location: 94.84
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGATTCCGGGGATCCGTCGACC and GATAATCGCGATAACTGACTTACGCATCGTTATTCCCCCTGACGCTGGTATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4132-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔhflK724::kan, hsdR514