Mutation ilvI784(del)::kan
- Name: ilvI784(del)::kan
- Type: Insertion
- Mutation of Gene: ilvI
- Approx. Map Location: 1.84
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGATTCCGGGGATCCGTCGACC and GAGTAAGACTGATAATATCCGGCGCATAATCAGGTTCTCTCCGTTTTGCTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0076-2 7 F-, Δ(araD-araB)567, ΔilvI784::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514