Mutation pstC758(del)::kan
- Name: pstC758(del)::kan
- Type: Insertion
- Mutation of Gene: pstC
- Approx. Map Location: 84.22
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GGCATTACAAAATGACTTTGTAAACGCGTTTAACTGAAGAGTAACTTATGATTCCGGGGATCCGTCGACC and CGCAGTGGTTTGCATTTCAACCATAGCCATTAGCGTGCCCCCTCATTCTTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3705-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔpstC758::kan, Δ(rhaD-rhaB)568, hsdR514