Mutation yadE747(del)::kan
- Name: yadE747(del)::kan
- Type: Insertion
- Mutation of Gene: yadE
- Approx. Map Location: 3.13
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGATTCCGGGGATCCGTCGACC and AAGCGATTCCGGTACAGGTTGCTTGATTGTTATCCCTGCGGCTGGTTACTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0126-1 7 F-, Δ(araD-araB)567, ΔyadE747::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514