Mutation ilvE721(del)::kan
- Name: ilvE721(del)::kan
- Type: Insertion
- Mutation of Gene: ilvE
- Approx. Map Location: 85.15
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GACGGTGCGTGCCGTCCCATTTTTTGTATTTATTGATTAACTTGATCTAAATTCCGGGGATCCGTCGACC and AACCACATCACAACAAATCCGCGCCTGAGCGCAAAAGGAATATAAAAATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5606-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔilvE721::kan, Δ(rhaD-rhaB)568, hsdR514