Mutation - polA5

Mutation - polA5Mutation polA5

Mutation polA5

Name: polA5
Type: Unknown
Mutation of Gene: polA
Approx. Map Location: 87.18
Comments
- polymerase altered pH opt., altered template specificity, lower overall activity; Exo-greater than normal levels. see G. and K.
References
- Grindley, ND, WS Kelley 1976. Effects of different alleles of the E. coli K12 pol A gene on the replication of non-transferring plasmids. Mol. Gen. Genet. 143(3):311-8.
- Matson, SW, FN Capaldo-Kimball, RA Bambara 1978. On the processive mechanism of Escherichia coli DNA Polymerase I. The polA5 mutation. J. Biol. Chem. 253(21):7851-6.
2 Strains Carrying This Mutation
Name Mutations Genotype
CM4050 6 F-, lacZ53(Am), λ^-, thyA36, rpsL151(strR), polA5, deoC2?
JG110 6 F-, λ^-, thyA36, IN(rrnD-rrnE)1, metE70, polA5, deoC2

Name	Mutations	Genotype
CM4050	6	F-, lacZ53(Am), λ^-, thyA36, rpsL151(strR), polA5, deoC2?
JG110	6	F-, λ^-, thyA36, IN(rrnD-rrnE)1, metE70, polA5, deoC2