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Mutation lexA3(Ind-)

• Name: lexA3(Ind-)
• Type: Base Pair Substitution
• Mutation of Gene: lexA
• Approx. Map Location: 91.71
• Properties

  Property	Symbol
  non-inducible	Ind-

  
  
• Comments
  • : Non-cleavable LexA repressor protein; defective in SOS induction, UV sensitive
  • This mutation results in a G85D amino acid substitution at the lexA cleavage site.
• References
  • Fernandez De Henestrosa, A.R., T. Ogi, S. Aoyagi, D. Chafin, J.J. Hayes, H. Ohmori, R. Woodgate 2000. Identification of additional genes belonging to the LexA regulon in Escherichia coli. Mol.Microbiol. 35:1560-1572
• 7 Strains Carrying This Mutation

  Name	Mutations	Genotype
  K996	13	Hfr(PO2A), fhuA22, phoA4(Am), λ-, ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, malE300::Tn10, lexA3(Ind-), mcrB1, creC510
  DM1415	16	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, tsx-33?, glnX44(AS), galK2(Oc), λ-, sulA211, recA1, rpsL31(strR), xylA5, mtl-1, thiE1?, lexA3(Ind-), lexA51
  DM1187	17	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, tsx-33?, glnX44(AS), galK2(Oc), λ-, sulA211, hisG4(Oc), recA441(ts), rpsL31(strR), xylA5, mtl-1, thiE1?, lexA51, lexA3(Ind-)
  DM936	18	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ-, Rac-0?, recA1, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), lexA41(ts), lexA3(Ind-)?
  DM1180	19	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, tsx-33?, glnX44(AS), galK2(Oc), λ-, sulA211, Rac-0, hisG4(Oc), rfbC1, mgl-51, recA441(ts), rpsL31(strR), kdgK51, xylA5, mtl-1, lexA3(Ind-)
  DM49	21	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1, lexA3(Ind-)
  JC11824	23	F-, thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ-, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpoS396(Am), rpsL31(strR), kdgK51, xylA5, mtl-1, rfa-308, argE3(Oc), thiE1, lexA3(Ind-)