E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pRL27

• Name: pRL27
• Type: Plasmid
  Plasmid Sites:

  oriT RP4

  kan

  oriR6Kgamma

  tetAp-tnp

  
  
• Priority: 0
• Comment:
  • pRL27 is a low-copy pir-dependent conjugative plasmid that encodes the kanamycin resistant hyperactive Tn5 system of Reznicoff.
• References:
  • Larsen, RA, MM Wilson, AM Guss, WW Metcalf 2002. Genetic analysis of pigment biosynthesis in Xanthobacter autotrophicus Py2 using a new, highly efficient transposon mutagenesis system that is functional in a wide variety of bacteria. Arch. Microbiol. 178(3):193-201.
  
  
• 1 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  BW20767/pRL27	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), leu-163::IS10, ΔuidA3::pir+, recA1, endA1, thiE1, hsdR17, creC510, pRL27