Site secB
- Name: secB
- Type: Gene
- Synonyms
b3609 MG1655 locus tag - Mnemonic: secretory (monitor)
- Left End Point: 81.51
- Right End Point: 81.52
- Products:
chaperone SecB - Direction: <
- Properties:
Property Comment encodes chaperone molecule for protein export - Priority: 1
- 2 Alleles of This Gene
secB721(del)::kan secB792-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank M24489 EcoGene.org EG10937 EcoliWiki secB - Comment:
- Protein export. Affects transloc. of MBP(maltose binding protein).Kinetic defect,but doesn't affect mal growth except in double mut.secB malE,which are Mal-.See Francetic.Enhances export OmpF,A,PhoE
- References:
- Kumamoto, C.A., J.R. Beckwith 1983. Mutations in a new gene, secB, cause defective porin localization in Escherichia coli. J.Bacteriol. 154:253-260
- Kumamoto, C.A., J.R. Beckwith 1985. Evidence for specificity at an early step in protein export in Escherichia coli. J.Bacteriol. 163:267-274
- Beall, B., J. Lutkenhaus 1987. Sequence analysis, transcriptional organization, and insertional mutagenesis of the envA gene of Escherichia coli. J.Bacteriol. 169:5408-5415
- Schmidt, M.G., E.E. Rollo, J. Grodberg, D.B. Oliver 1988. Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli. J.Bacteriol. 170:3404-3414
- Lecker, S., R. Lill, T. Ziegelhoffer, C. Georgopoulos, P.J. Bassford, C.A. Kumamoto, W. Wickner 1989. Three pure chaperone proteins of Escherichia coli--SecB, trigger factor and GroEL--form soluble complexes with precursor proteins in vitro. EMBO J. 8:
- Watanabe, M., G. Blobel 1989. SecB functions as a cytosolic signal recognition factor for protein export in E. coli. Cell 58:695-705
- Kumamoto, C.A. 1991. Molecular chaperone and protein translocation across the Escherichia coli inner membrane. Mol.Microbiol. 5:19-22
- Kim, J., Y. Lee, C. Kim, C. Park 1992. Involvement of SecB, a chaperone, in the export of ribose-binding protein. J.Bacteriol. 174:5219-5227
- Francetic, O., M.P. Hanson, C.A. Kumamoto 1993. prlA supression of defective export of maltose-binding protein in secB mutants of Escherichia coli. J.Bacteriol. 175:4036-4044
- Watanabe, M., G. Blobel 1995. High-affinity binding of Escherichia coli SecB to the signal sequence region of a presecretory protein. Proc.Natl.Acad.Sci.USA 92:10133-10136
- Kimsey, H.H., M.D. Dagarag, C.A. Kumamoto 1995. Diverse effects of mutation on the activity of the Escherichia coli export chaperone SecB. J.Biol.Chem. 270:22831-22835
- Powers, E.L., L.L. Randall 1995. Export of periplasmic galactose-binding protein in Escherichia coli depends on the chaperone SecB. J.Bacteriol. 177:1906-1907
- Van Raalte, A.L., R.A. Demel, G. Verberkmoes, E. Breukink, R.C. Keller, B. De Kruijff 1996. Influence of the signal sequence and chaperone SecB on the interaction between precursor protein prePhoE and phospholipids. Eur.J.Biochem. 235:207-214