E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home:
• Scope of the Collection:
• The Database
• Acknowledgment of Support

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help

How to Request Strains or Information

• Contact Information
• Charges
• Strains Not Found?

Other CGSC Information

• FAQ on Procedures
• Current Working Map
• 1998 MMBR Map
• 1998 MMBR Gene List
• Map Diagrams (PDFs)

Other Links

• E. coli Links
• Other Stock Collections
• Other Bio Links
• Yale Web

Funded by a grant from the National Science Foundation

Site hlpA

• Name: hlpA
• Type: Gene
  Synonyms

  b0178	MG1655 locus tag
  firA
  ompH	Salmonella map
  orf(skp)
  skp	Orf upstream from firA, see Dicker, Thome refs

  
  
• Mnemonic: histone-like protein
• Left End Point: 4.32
• Right End Point: 4.33
  Products:

  histone-like protein HLP-I (cytoplasmic?)

  OmpH, outer membrane protein

  
  
• Direction: >
• Priority: 1
  2 Alleles of This Gene

  hlpA781(del)::kan

  hlpA791-YFP(::cat)

  
  
• External Database Links:

  Host Site	Page Links
  EcoGene.org	EG10455
  EcoliWiki	hlpA

  
  
• Comment:
  • histone-like protein HLP-I (BH1); DNA-binding. But also, OmpH homolog (see Hirvas ref.) hlpA = skp = firA. Possible chaperone or folding catalyst in extracytoplasmic compartments
• References:
  • Lathe, R., H. Buc, J.-P. Lecocq, E.K.F. Bautz 1980. Prokaryotic histone-like protein interacting with RNA polymerase. Proc.Natl.Acad.Sci.USA 77:3548-3552
  
  
  • Aasland, R.J., J. Coleman, A.L. Holck, C.L. Smith, C.R.H. Raetz, K. Kleppe 1988. Identity of the 17-kilodalton protein, a DNA-binding protein from Escherichia coli, and the firA gene product. J.Bacteriol. 170:5916-5918
  
  
  • Coleman, J., C.R.H. Raetz 1988. First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene. J.Bacteriol. 170:1268-1274
  
  
  • Holck, A.L., K. Kleppe 1988. Cloning and sequencing of the gene for the DNA-binding 17K protein of Escherichia coli. Gene 67:117-124
  
  
  • Hirvas, L., J. Coleman, P. Koski, M. Vaara 1990. Bacterial "histone-like protein I" (HLP-I) is an outer membrane constituent?. FEBS Lett. 262:123-126
  
  
  • Thome, B.M., H.K. Hoffschulte, E. Schiltz, M. Muller 1990. A protein with sequence identity to Skp (FirA) supports protein translocation into plasma membrane vesicles of Escherichia coli. FEBS Lett. 269:113-116
  
  
  • Hirvas, L., P. Koski, M. Vaara 1991. The ompH gene of Yersinia enterocolitica: cloning, sequencing, expression, and comparison with known enterobacterial ompH sequences. J.Bacteriol. 173:1223-1229
  
  
  • Dicker, I.B., S. Seetharam 1991. Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J.Bacteriol. 173:334-344
  
  
  • Thome, B.M., M. Muller 1991. Skp is a periplasmic Escherichia coli protein requiring SecA and SecY for export. Mol.Microbiol. 5:2815-2821
  
  
  • Helander, I.M., B. Lindner, U. Seydel, M. Vaara 1993. Defective biosynthesis of the lipid A component of temperature-sensitive firA (omsA) mutant of Escherichia coli. Eur.J.Biochem. 212:363-369
  
  
  • Chen, R., U. Henning 1996. A periplasmic protein (Skp) of Escherichia coli selectively binds a class of outer membrane proteins. Mol.Microbiol. 19:1287-1294
  
  
  • Missiakas, D., J.M. Betton, S. Raina 1996. New components of protein folding in extracytoplasmic compartments of Escherichia coli SurA, FkpA and Skp/OmpH. Mol.Microbiol. 21:871-884