Site rpoB
- Name: rpoB
- Type: Gene
- Synonyms
b3987 MG1655 locus tag ftsR ftsR1 for nonlethal filamentation allele (Vinella & D'Ari groN nitB rif ron sdgB stl stv tabD - Mnemonic: RNA polymerase
- Left End Point: 90.08
- Right End Point: 90.16
- Products:
RNA polymerase, beta subunit - Direction: >
- Properties:
Property Comment rifamycin resistance NULL - Priority: 1
- External Database Links:
Host Site Page Links EcoGene.org EG10894 EcoliWiki rpoB - Comment:
- Sel.phen. Streptovaricin res; rifampicin res.; streptolydigin res.; fluorouracil res.; large col. on plate with phage LAM and phage 434 mutants; phage LAM Nmar mutant res.; in relA-: L- serine res.
- References:
- Yura, T., K. Igarishi 1968. RNA polymerase mutants of Escherichia coli. I. Mutants resistant to streptovaricin. Proc.Natl.Acad.Sci.USA 61:1313-1319
- Ezekiel, D.H., J.E. Hutchins 1968. Mutations affecting RNA polymerase associated with rifampicin resistance in Escherichia coli. Nature 220:276-277
- Schleif, R. 1969. Isolation and characterization of a streptolydigin resistant RNA polymerase. Nature 223:1068-1069
- Austin, S., J. G. Scaife 1970. A new method for selecting RNA polymerase mutants. J.Mol.Biol. 49:263-267
- Georgopoulos, C. 1971. Bacterial mutants in which the gene N function of bacteriophage lambda is blocked have an altered RNA polymerase. Proc.Natl.Acad.Sci.USA 68:2977-2981
- Ghysen, A., M. Pirionio 1972. Relationship between the N function of bacteriophage LAM and host RNA polymerase. J.Mol.Biol. 65:259-272
- Austin, S. 1976. Wild-type and mutant in vitro products of an operon for ribonucleic acid polymerase subunits. J.Bacteriol. 127:32-39
- Uzan, M., A. Danchin 1978. Correlation between the serine sensitivity and the derepressibility of the ilv genes in Escherichia coli relA- mutants. Mol.Gen.Genet. 165:21-30
- Barry, G., C.L. Squires, C. Squires 1979. Control features within the rplJL-rpoBC transcription unit of Escherichia coli. Proc.Natl.Acad.Sci.USA 76:4922-4926
- Linn, T., M. Goman, J. G. Scaife 1979. Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome. J.Bacteriol. 140:479-489
- Barry, G., C. Squires, C.L. Squires 1980. Attentuation and processing of RNA from the rplJL-rpoBC transcription unit of Escherichia coli. Proc.Natl.Acad.Sci.USA 77:3331-3335
- Kajitani, M., R. Fukuda, A. Ishihama 1980. Autogenous and post-translational regulation of Escherichia coli RNA polymerase synthesis in vitro. Mol.Gen.Genet. 179:489-496
- Delcuve, G., W.L. Downing, H.M. Lewis, P.P. Dennis 1980. Nucleotide sequence of the proximal portion of the RNA polymerase B subunit gene of Escherichia coli. Gene 11:367-373
- An, G., J.D. Friesen 1980. Characterization of promoter-cloning plasmids: analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced promoter mutant. J.Bacteriol. 144:904-916
- Newman, A.J., R.S. Hayward 1980. Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12. Mol.Gen.Genet. 177:527-533
- Holowachuk, E.W., J.D. Friesen, N. Fiil 1980. Bacteriophage lambda vehicle for the direct cloning of Escherichia coli promoter DNA sequences: feedback regulation of the rplJL-rpoBC operon. Proc.Natl.Acad.Sci.USA 77:2124-2128
- Little, R., N. Fiil, P.P. Dennis 1981. Transcription and post-transcriptional control of ribosomal protein and ribonucleic acid polymerase genes. J.Bacteriol. 147:25-35
- Bruckner, R., H. Matzura 1981. In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli. Mol.Gen.Genet. 183:277-282
- Ma, J.-C., A.J. Newman, R.S. Hayward 1981. Internal promoters of the rpoBC operon of Escherichia coli. Mol.Gen.Genet. 184:548-550
- Ovchinnikov, Y.A., G.S. Monastyrskaya, V.V. Gubanov, S.O. Guryev, O.Y. Chertov, N.N. Modyanov, V.A. Grinkevich, I.A. Makarova, T.V. Marchenko, I.N. Polovnikova, V.M. Lipkin, E.D. Sverdlov 1982. The primary structure of Escherichia coli RNA polymerase.
- Hui, I., K. Maltman, R. Little, S. Hastrup, M. Johnsen, N. Fiil, P.P. Dennis 1982. Insertions of transposon Tn5 into ribosomal protein RNA polymerase operons. J.Bacteriol. 152:1022-1032
- Ralling, G., T. Linn 1984. Relative activities of the transcriptional regulatory sites in the rplKAJLrpoBC gene cluster of Escherichia coli. J.Bacteriol. 158:279-285
- Downing, W.L., P.P. Dennis 1987. Transcription products from the rplKAJL-rpoBC gene cluster. J.Mol.Biol. 194:609-620
- Jin, D.J., C.A. Gross 1989. Three rpoBC mutations that suppress the termination defects of rho mutants also affect the functions of nusA mutants. Mol.Gen.Genet. 216:269-275
- Lee, J., M. Kashlev, S. Borukhov, A. Goldfarb 1991. A beta subunit mutation disrupting the catalytic function of Escherichia coli RNA polymerase. Proc.Natl.Acad.Sci.USA 88:6018-6022
- Steward, K.L., T. Linn 1991. In vivo analysis of overlapping transcription units in the rplKAJLrpoBC ribosomal protein-RNA polymerase gene cluster of Escherichia coli. J.Mol.Biol. 218:23-31
- Rockwell, P., M.E. Gottesman 1991. An Escherichia coli rpoB mutation that inhibits transcription of catabolite-sensitive operons. J.Mol.Biol. 222:189-196
- Steward, K.L., T. Linn 1992. Transcription frequency modulates the efficiency of an attenuator preceding the rpoBC RNA polymerase genes of Escherichia coli: possible autogenous control. Nucleic Acids Res. 20:4773-4779
- Kogoma, T. 1994. Escherichia coli RNA polymerase mutants that enhance or diminish the SOS response constitutively expressed in the absence of RNase H1 activity. J.Bacteriol. 176:1521-1523
- Vinella, D., R. D'Ari 1994. Thermoinducible filamentation in Escherichia coli due to an altered RNA polymerase beta subunit is suppressed by high levels of ppGpp. J.Bacteriol. 176:966-972
- Rowland, G.C., P.P. Lim, R.E. Glass 1995. In vivo cloning of a carboxy-terminal rpoB allele which confers altered transcriptional properties. Folia Microbiol (Praha) 40:588-594
- Dykxhoorn, D.M., R. St Pierre, T. Linn 1996. Synthesis of the beta and beta' subunits of Escherichia coli RNA polymerase is autogenously regulated in vivo by both transcriptional and translational mechanisms. Mol.Microbiol. 19:483-493
- Zhou, Y.N., D.J. Jin 1998. The rpoB mutants destabilizing initiation complexes at stringently controlled promoters behave like "stringent" RNA polymerases in Escherichia coli. Proc.Natl.Acad.Sci.USA 95:2908-2913
- Esteban-Gamboa, A., J. Balzarini, R. Esnouf, E. De Clercq, M.J. Camarasa, M.J. Perez-Perez 2000. Design, synthesis, and enzymatic evaluation of multisubstrate analogue inhibitors of Escherichia coli thymidine phosphorylase. J Med Chem 43:971-983