Site priA
- Name: priA
- Type: Gene
- Synonyms
b3935 MG1655 locus tag srgA suppressor of recG mutations - Mnemonic: primase
- Left End Point: 88.86
- Right End Point: 88.90
- Products:
primosome factor Y a.k.a. protein n' primosome protein n' - Direction: <
- Properties:
Property Comment SOS-related mutation induces SOS DNA biosynthesis primosomal assembly; absence thought to stall or destabilize replication fork encodes DNA-dependent ATPase primosome assembly site(PAS)-sequence-dependent dATPase encodes helicase 3'-5' helicase encodes NA-binding protein primsome-assembly-site(PAS)-sequence-specific DNA-binding - Priority: 0
- 2 Alleles of This Gene
priA1::KanR priA722(del)::kan - External Database Links:
Host Site Page Links NCBI/GenBank M33293 EcoGene.org EG10763 EcoliWiki priA - Comment:
- New gene 1990
- References:
- Nurse, P., R.J. DiGate, K.H. Zavitz, K.J. Marians 1990. Molecular cloning and DNA sequence analysis of Escherichia coli priA, the gene encoding the primosomal protein replication factor Y. Proc.Natl.Acad.Sci.USA 87:4615-4619
- Lee, E.H., H. Masai, G.C. Allen, A. Kornberg 1990. The priA gene encoding the primosomal replicative n' protein of Escherichia coli. Proc.Natl.Acad.Sci.USA 87:4620-4624
- Ouzounis, C.A., B.J. Blencowe 1991. Bacterial DNA replication initiation factor priA is related to proteins belonging to the 'DEAD-box' family. Nucleic Acids Res. 19:6953
- Nurse, P., K.H. Zavitz, K.J. Marians 1991. Inactivation of the Escherichia coli PriA DNA replication protein induces the SOS response. J.Bacteriol. 173:6686-6693
- Sandler, S.J. 1996. Overlapping functions for recF and priA in cell viability and UV-inducible SOS expression are distinguished by dnaC809 in Escherichia coli K-12. Mol.Microbiol. 19:871-880
- Al-Deib, A.A., A.A. Mahdi, R.G. Lloyd 1996. Modulation of recombination and DNA repair by the RecG and PriA helicases of Escherichia coli K-12. J.Bacteriol. 178:6782-6789
- Liu, J, L Xu, SJ Sandler, KJ Marians 1999. Replication fork assembly at recombination intermediates is required for bacterial growth. Proc. Natl. Acad. Sci. U.S.A. 96(7):3552-5.
- Xu, L., K.J. Marians 2000. Purification and characterization of DnaC810, a primosomal protein capable of bypassing PriA function. J.Biol.Chem. 275:8196-8205
- Cadman, C.J., P. McGlynn 2004. PriA helicase and SSB interact physically and functionally. Nucleic Acids Res. 32:6378-6387
- Cadman, C.J., M. Lopper, P.B. Moon, J.L. Keck, P. McGlynn 2005. PriB stimulates PriA helicase via an interaction with single-stranded DNA. J.Biol.Chem. 280:39693-39700