Site recB
- Name: recB
- Type: Gene
- Synonyms
b2820 MG1655 locus tag rorA - Mnemonic: Recombination
- Left End Point: 63.60
- Right End Point: 63.68
- Products:
RecBCD enzyme subunit exonuclease V subunit exonuclease/helicase - Direction: <
- Priority: 1
- 9 Alleles of This Gene
recB1067(D1067A) recB1080(D1080A) recB1082(K1082Q) recB1910(del)::dhfr recB21 recB270(ts) recB60 recB745(del)::kan recB791-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank X04581 EcoGene.org EG10824 EcoliWiki recB - Comment:
- Muts are def. for homologous recomb., sensitive to DNA=damaging agents and unable to restrict foreign DNA and produce inviable cells in culture. RecBCD req. for MMS repair, not for recomb.
- References:
- Hickson, I.D., P.T. Emmerson 1981. Identification of the Escherichia coli recB and recC gene products. Nature 294:578-580
- Sasaki, M., T. Fujiyoshi, K. Shimada, Y. Takagi 1982. Fine structure of the recB and recC gene region of Escherichia coli. Biochem.Biophys.Res.Commun. 109:414-422
- Dykstra, C.C., D. Prasher, S.R. Kushner 1984. Physical and biochemical analysis of the cloned recB and recC genes of Escherichia coli K-12. J.Bacteriol. 157:21-27
- Chaudhury, A.M., G.R. Smith 1985. Role of Escherichia coli RecBC enzyme in SOS induction. Mol.Gen.Genet. 201:525-528
- Finch, P.W., A. Storey, K.E. Chapman, K. Brown, I.D. Hickson, P.T. Emmerson 1986. Complete nucleotide sequence of the Escherichia coli recB gene. Nucleic Acids Res. 14:8573-8582
- Clark, A.J. 1991. rec genes and homologous recombination proteins in Escherichia coli. Biochimie 73:523-532
- Estevenon, A.M., J. Kooistra, N. Sicard 1995. An Escherichia coli strain deficient for both exonuclease V and deoxycytidine triphosphate deaminase shows enhanced sensitivity to ionizing radiation. Mol.Gen.Genet. 246:514-518
- Taylor, A.F., G.R. Smith 1995. Monomeric RecBCD enzyme binds and unwinds DNA. J.Biol.Chem. 270:24451-24458
- Taylor, A.F., G.R. Smith 1995. Strand specificity of nicking of DNA at Chi sites by RecBCD enzyme. Modulation by ATP and magnesium levels. J.Biol.Chem. 270:24459-24467
- Bianco, P.R., S.C. Kowalczykowski 1997. The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3'. Proc.Natl.Acad.Sci.USA 94:6706-6711
- Poteete, A.R., A.C. Fenton 2000. Genetic requirements of phage lambda red-mediated gene replacement in Escherichia coli K-12. J.Bacteriol. 182:2336-2340