Site dnaE
- Name: dnaE
- Type: Gene
- Synonyms
b0184 MG1655 locus tag polC - Mnemonic: DNA
- Left End Point: 4.42
- Right End Point: 4.50
- Products:
DNA polymerase III, alpha subunit - Direction: >
- Priority: 1
- 7 Alleles of This Gene
dnaE1026(ts) dnaE1044 dnaE1061 dnaE1126 dnaE486(ts) dnaE74(ts) dnaE793-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank M96394 EcoGene.org EG10238 EcoliWiki dnaE - Comment:
- The polymerase (subu.) that selects correct nucleotides during template-directed synthesis. (acts as antimutator in mutL- and mutT- strains)
- References:
- Bendiak, D.S., J.D. Friesen 1981. Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed. Mol.Gen.Genet. 181:356-362
- Welch, M.M., C.S. McHenry 1982. Cloning and identification of the product of the dnaE gene of Escherichia coli. J.Bacteriol. 152:351-356
- Shepard, D., R.W. Oberfelder, M.M. Welch, C.S. McHenry 1984. Determination of the precise location and orientation of the Escherichia coli dnaE gene. J.Bacteriol. 158:455-459
- Tomasiewicz, H.G., C.S. McHenry 1987. Sequence analysis of the Escherichia coli dnaE gene. J.Bacteriol. 169:5735-5744
- Witkin, E.M., V. Roegner-Maniscalco 1992. Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I. J.Bacteriol. 174:4166-4168
- Oller, A.R., I.J. Fijalkowska, R.M. Schaaper 1993. The Escherichia coli galK2 papillation assay: its specificity and application to seven newly isolated mutator strains. Mutat.Res. 292:175-185
- Fijalkowska, I.J., R.M. Schaaper 1995. Effects of Escherichia coli dnaE antimutator alleles in a proofreading-deficient mutD5 strain. J.Bacteriol. 177:5979-5986
- Kelman, Z., M.A. O'Donnell 1995. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine. Annu.Rev.Biochem. 64:171-200
- Schaaper, R.M. 1996. Suppressors of Escherichia coli mutT: anitimutators for DNA replication errors. Mutat.Res. 350:17-23
- Tomer, G., O. Cohen-Fix, M.A. O'Donnell, M. Goodman, Z. Livneh 1996. Reconstitution of repair-gap UV mutagenesis with purified proteins from Escherichia coli: a role for DNA polymerases III and II. Proc.Natl.Acad.Sci.USA 93:1376-1380
- Fijalkowska, I.J., R.L. Dunn, R.M. Schaaper 1997. Genetic requirements and mutational specificity of the Escherichia coli SOS mutator activity. J.Bacteriol. 179:7435-7445
- Jonczyk, P., A. Nowicka, I.J. Fijalkowska, R.M. Schaaper, Z. Ciesla 1998. In vivo protein interactions within the Escherichia coli DNA polymerase III core. J.Bacteriol. 180:1563-1566