Site polA
- Name: polA
- Type: Gene
- Synonyms
b3863 MG1655 locus tag resA - Mnemonic: polymerase
- Left End Point: 87.18
- Right End Point: 87.24
- Products:
DNA polymerase I - Direction: >
- Priority: 1
- 16 Alleles of This Gene
polA0 polA1(Am) polA107 polA11 polA12(ts) polA20 polA34(ts) polA4113(ts) polA480(ts,EX) polA5 polA546(ts,EX) polA591 polA6 polA726(del)::kan polA791-YFP(::cat) polA792-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank J01663 NCBI/GenBank V00317 EcoGene.org EG10746 EcoliWiki polA - Comment:
- Selectable phenotype.Loss: resist.to phage LAM gamma- mutants. Note also:Replic.of plasmids derived from colE1 requires polA prod. Without PolA, sel.gives plasm.marker incorp. Promotes strand exchange
- References:
- Zissler, J., E. Signer, F. Schaefer 0. The role of recombination in growth of bacteriophage lambda. I. The gamma gene, p. 455-468. In A.D. Hershey (ed.), The bacteriophage lambda. In: The Bacteriophage Lambda
- Kelley, W.S., H.J. Whitfield, Jr. 1971. Purification of an altered DNA polymerase from an E. coli strain with a pol mutation. Nature 230:33-36
- Murray, N.E., W.S. Kelley 1979. Characterization of lambda-polA transducing phages: effective expression of the E. coli polA gene. Mol.Gen.Genet. 175:77-87
- Kelley, W.S. 1980. Mapping of the polA locus of Escherichia coli K12: genetic fine structure of the cistron. Genetics 95:15-38
- Joyce, C.M., W.S. Kelley, N. Grindley 1982. Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. J.Biol.Chem. 257:1958-1964
- Yang, Y.-L., B. Polisky 1993. Suppression of ColE1 high-copy-number mutants by mutations in the polA gene of Escherichia coli. J.Bacteriol. 175:428-437
- Zhang, W., D.H. Evans 1995. DNA strand transfer catalyzed by the 5'-3' exonuclease domain of Escherichia coli DNA polymerase I. Nucleic Acids Res. 23:4620-4627
- Tuske, S., K. Singh, N. Kaushik, M.J. Modak 2000. The J-helix of Escherichia coli DNA polymerase I (Klenow fragment) regulates polymerase and 3'- 5'-exonuclease functions. J.Biol.Chem. 275:23759-68
- Nagata, Y., K. Mashimo, M. Kawata, K. Yamamoto 2002. The roles of Klenow processing and flap processing activities of DNA polymerase I in chromosome instability in Escherichia coli K12 strains. Genetics 160:13-23