Site lon
- Name: lon
- Type: Gene
- Synonyms
b0439 MG1655 locus tag capR deg dir muc - Mnemonic: Long form
- Left End Point: 9.88
- Right End Point: 9.93
- Products:
DNA-binding, ATP-dependent protease LA, cytoplasmic - Direction: >
- Properties:
Property Comment encodes a protease NULL heat shock regulon NULL - Priority: 1
- 14 Alleles of This Gene
lon-10 lon-100(del?) lon-11 lon-1::IS186 lon-20 lon-201 lon-202 lon-21 lon-22 lon-6 lon-7 lon-725(del)::kan lon-791-YFP(::cat) lon-9 - External Database Links:
Host Site Page Links NCBI/GenBank J03896 EcoGene.org EG10542 EcoliWiki lon - Comment:
- E. coli B is lon-minus: Greenberg, Mut.Res.2:304. Selectable phenotype. Chlorpromazine, promethazine resistance. Site-specific DNA binding affecting many cell functions
- References:
- Markovitz, A. 1964. Regulatory mechanism for synthesis of capsular polysaccharide in mucoid mutants of Escherichia coli K-12. Proc.Natl.Acad.Sci.USA 51:239-240
- Markovitz, A., N. Rosenbaum 1965. A regulator gene that is dominant on an episome and recessive on a chromosome. Proc.Natl.Acad.Sci.USA 54:1084-1091
- Adler, H.I., W.D. Fisher, A.A. Hardigree, G.E. Stapleton 1966. Repair of radiation-induced damage to the cell division mechanism of Escherichia coli. J.Bacteriol. 91:737-742
- Donch, J., J. Greenberg 1968. Genetic analysis of lon mutants of strain K-12 of Escherichia coli. Mol.Gen.Genet. 103:105-115
- Gayda, R.C., L.T. Yamamoto, A. Markovitz 1976. Second-site mutations in capR (lon) strains of Escherichia coli K-12 that prevent radiation sensitivity and allow bacteriophage lambda to lysogenize. J.Bacteriol. 127:1208-1216
- Molnar, J., I.B. Holland, Y. Mandi 1977. Selection of lon mutants in Escherichia coli by treatment with phenothiazines. Genet.Res. 30:13-20
- Zehnbauer, B.A., A. Markovitz 1980. Cloning of gene lon (capR) of Escherichia coli K-12 and identification of polypeptides specified by the cloned deoxyribonucleic acid fragment. J.Bacteriol. 143:852-863
- Zehnbauer, B.A., E.C. Foley, G.W. Henderson, A. Markovitz 1981. Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein. Proc.Natl.Acad.Sci.USA 78:2043-2047
- Schoemaker, J.M., A. Markovitz 1981. Identification of the gene lon (capR) product as a 94-kilodalton polypeptide by cloning and deletion analysis. J.Bacteriol. 147:46-56
- Chung, C.H., A.L. Goldberg 1981. The product of the lon (capR) gene in Escherichia coli is the ATP-dependent protease, protease La. Proc.Natl.Acad.Sci.USA 78:4931-4935
- Charette, M.F., G.W. Henderson, A. Markovitz 1981. ATP hydrolysis-dependent protease activity of the lon (capR) protein of Escherichia coli K-12. Proc.Natl.Acad.Sci.USA 78:4728-4732
- Gayda, R.C., P.E. Stephens, R. Hewick, J.M. Schoemaker, W.J. Dreyer, A. Markovitz 1985. Regulatory region of the heat shock-inducible capR (lon) gene: DNA and protein sequences. J.Bacteriol. 162:271-275
- Maurizi, M.R., P. Trisler, S. Gottesman 1985. Insertional mutagenesis of the lon gene in Escherichia coli: lon is dispensable. J.Bacteriol. 164:1124-1135
- Chin, D.T., S.A. Goff, T. Webster, T. Smith, A.L. Goldberg 1988. Sequence of the lon gene in Escherichia coli.A heat-shock gene which encodes the ATP-dependent protease La. J.Biol.Chem. 263:11718-11728
- Thomas, C.D., J. Modha, T.M. Razzaq, P.M. Cullis, A.J. Rivett 1993. Controlled high-level expression of the lon gene of Escherichia coli allows overproduction of Lon protease. Gene 136:237-242
- Frank, E.G., D.G. Ennis, M. Gonzalez, A.S. Levine, R. Woodgate 1996. Regulation of SOS mutagenesis by proteolysis. Proc.Natl.Acad.Sci.USA 93:10291-10296
- Fu, G.K., M.J. Smith, D.M. Markovitz 1997. Bacterial protease Lon is a site-specific DNA-binding protein. J.Biol.Chem. 272:534-538