Site tufB
- Name: tufB
- Type: Gene
- Synonyms
b3980 MG1655 locus tag kirT kirromycin resistant alleles pulT pulvomycin resistant alleles - Mnemonic: Tu (Elongation) factor, EF-Tu
- Left End Point: 89.96
- Right End Point: 89.99
- Products:
EF-Tu subunit elongation factor-T(translation)u(unstable) - Direction: >
- Properties:
Property Comment kirromycin,pulvomycinR alleles NULL duplicate gene see tufA - Priority: 1
- 2 Alleles of This Gene
tufB756(del)::kan tufB792-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank M29107 NCBI/GenBank X04176 EcoGene.org EG11037 EcoliWiki tufB - Comment:
- Selectable phenotype. Kirromycin resistance. Some KirR alleles reverse strep-dependent phenotype of rps protein S12 to strep sensitivity.
- References:
- Van de Klundert, J.A., P.H. Van der Meide, P. Van de Putte, L. Bosch 1978. Mutants of Escherichia coli altered in both genes coding for the elongation factor Tu. Proc.Natl.Acad.Sci.USA 75:4470-4473
- Taylor, W.E., R.R. Burgess 1979. Escherichia coli RNA polymerase binding and initiation of transcription on fragments of lambda rif^d 18 DNA containing promoters for lambda genes and for rrnB, tufB, rplC,A, rplJ,L and rpoB,C genes. Gene 6:331-365
- Linn, T., M. Goman, J. G. Scaife 1979. Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome. J.Bacteriol. 140:479-489
- An, G., J.D. Friesen 1980. Characterization of promoter-cloning plasmids: analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced promoter mutant. J.Bacteriol. 144:904-916
- An, G., J.D. Friesen 1980. The nucleotide sequence of tufB and four nearby tRNA structural genes for Escherichia coli. Gene 12:33-39
- Miyajima, A., M. Shibuya, Y. Kuchino, Y. Kaziro 1981. Transcription of the E. coli tufB gene: cotranscription with four tRNA genes and inhibition by guanosine-5'-diphosphate-3'-diphosphate. Mol.Gen.Genet. 183:13-19
- Laursen, R.A., J.J. L'Italien, S. Nagarkatti, D.L. Miller 1981. The amino acid sequence of elongation factor Tu of Escherichia coli.The complete sequence. J.Biol.Chem. 256:8102-8109
- Lee, J.S., G. An, J.D. Friesen, N. Fiil 1981. Location of the tufB promoter of E. coli: cotranscription of tufB with four transfer RNA genes. Cell 25:251-258
- Hudson, L., J.J. Rossi, A. Landy 1981. Dual function transcripts specifying tRNA and mRNA. Nature 294:422-427
- Takebe, Y., Y. Kaziro 1982. In vitro construction of the tufB-lacZ fusion: analysis of the regulatory mechanism of tufB promoter. Mol.Gen.Genet. 187:355-363
- Van der Meide, P.H., E. Vijgenboom, M. Dicke, L. Bosch 1982. Regulation of the expression of tufA and tufB, the two genes coding for elongation factor EF-Tu in Escherichia coli. FEBS Lett. 139:325-330
- van Delft, J.H., B. Marinon, D.S. Schmidt, L. Bosch 1987. Transcription of the tRNA-tufB operon of Escherichia coli: activation, termination and antitermination. Nucleic Acids Res. 15:9515-9530
- Verbeek, H., L. Nilssoj, L. Bosch 1992. The mechanism of trans-activation of the Escherichia coli operon thrU(tufB) by the protein FIS - a model. Nucleic Acids Res. 20:4077
- Kraal, B., L.A. Zeef, J.R. Mesters, K. Boon, E.L. Vorstenbosch, L. Bosch, P.H. Anborgh, A. Parmeggiani, R. Hilgenfeld 1995. Antibiotic resistance mechanisms of mutant EF-Tu species in Escherichia coli. Biochem.Cell Biol. 73:1167-1177
- Guillon, J.M., S. Heiss, J. Soutourina, Y. Mechulam, S. Laalami, M. Grunberg-Manago, S. Blanquet 1996. Interplay of methionine tRNAs with translation elongation factor Tu and translation initiation factor 2 in Escherichia coli. J.Biol.Chem. 271:22321-2
- Wiborg, O., C. Andersen, C.R. Knudsen, B.F.C. Clark, J. Nyborg 1996. Mapping Escherichia coli elongation factor Tu residues involved in binding of aminoacyl-tRNA. J.Biol.Chem. 271:20406-20411
- Talens, A., K. Boon, B. Kraal, L. Bosch 1996. Translational activities of EF-Tu [G222D] which cannot be reconciled with the classical scheme of the polypeptide chain elongation cycle. Biochem.Biophys.Res.Commun. 225:961-967