E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site pKD46

• Name: pKD46
• Type: Plasmid
  SubSites:

  araC

  bla

  [tL3]

  
  
  Plasmid Sites:

  repA101(ts)

  araBp-gam-bet-exo

  oriR101

  bla(ApR)

  
  
• Priority: 0
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	AY048746

  
  
• Comment:
  • Lambda Red recombinase expression plasmid -- includes 2154 nucleotides (31088-33241) of phage Lambda, which includes the terminator downstream frp, exo, tL3 and a small ORF.GenBk-AY048746
• References:
  • Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
  
  
• 3 Strains Carrying This Plasmid

  Name	Mutations	Genotype
  MG1655/pKD46	2	F-, λ-, rph-1, pKD46
  BW25113/pKD46	6	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD46
  BW25141/pKD46	11	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, λ-, galU95, ΔuidA3::pir+, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514, pKD46