Site gyrB
- Name: gyrB
- Type: Gene
- Synonyms
acrB b3699 MG1655 locus tag Cou himB hisU nalC nalD pcbA - Mnemonic: Gyrase
- Left End Point: 83.53
- Right End Point: 83.59
- Products:
DNA gyrase, subunit B DNA topoisomerase (ATP-hydrolysing) - Direction: <
- Priority: 1
- 7 Alleles of This Gene
gyrB203(ts) gyrB204 gyrB221(CouR) gyrB225 gyrB226 gyrB41(Const) gyrB792-YFP(::cat) - External Database Links:
Host Site Page Links EcoGene.org EG10424 EcoliWiki gyrB - Comment:
- Selectable phenotype. Novobiocin, coumermycin resistance; chlorobiocin resistance; growth on B-glucosides, stronger growth of topA-; survival of infection with mutant phage LAM.
- References:
- Gellert, M., M.H. O'Dea, A. Itoh, J.-I. Tomizawa 1976. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc.Natl.Acad.Sci.USA 73:4474-4478
- Miller, H.I., Y. Kikuchi, H.A. Nash, R. Weisberg, D.I. Friedman 1978. Site-specific recombination of bacteriophage lambda: the role of host gene products. Cold Spr.Harb.Symp.Quant.Biol. 43:1121-1126
- Orr, G.A., N.F. Fairweather, I.B. Holland, R.H. Pritchard 1979. Isolation and characterization of a strain carrying a conditional lethal mutation in the cou gene of Escherichia coli K-12. Mol.Gen.Genet. 177:103-112
- von Meyenburg, K., F.G. Hansen, E. Riise, H.E.N. Bergmans, M. Meijer, W. Messer 1980. The origin of replication, oriC, of the Escherichia coli chromosome: genes near oriC and construction of oriC deletion mutations. In B. Alberts, and C.F. Fox (ed),
- DiNardo, S., K.A. Voelkel, R. Sternglanz, A.E. Reynolds, A. Wright 1982. Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes. Cell 31:43-51
- Adachi, T., R. Mizuuchi, R. Menzel, M. Gellert 1984. DNA sequence and transcription of the region upstream of the E. coli gyrB gene. Nucleic Acids Res. 12:6389-6395
- Yamagishi, J.-I., K. Yoshida, M. Yamayoshi, S. Nakamura 1986. Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli. Mol.Gen.Genet. 204:367-373
- Menzel, R., M. Gellert 1987. Modulation of transcription by DNA supercoiling: a deletion analysis of the Escherichia coli gyrA and gyrB promoters. Proc.Natl.Acad.Sci.USA 84:4185-4189
- Menzel, R., M. Gellert 1987. Fusions of the Escherichia coli gyrA and gyrB control regions to the galactokinase gene are inducible by coumermycin treatment. J.Bacteriol. 169:1272-1278
- Adachi, T., M. Mizuuchi, A. Robinson, E. Appella, M.H. O'Dea, M. Gellert, K. Mizuuchi 1987. DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy. Nucleic Acids Res. 15:771-784
- Nakamura, S., M. Nakamura, T. Kojima, H. Yoshida 1989. gyrA and gyrB mutations in quinolone-resistant strains of Escherichia coli. Antimicrob.Agents Chemother. 33:254-255
- von Freiesleben, U. 1991. DNA replication in Escherichia coli gyrB(Ts) mutants analysed by flow cytometry. Res.Microbiol. 142:223-227
- Toone, W.M., K.E. Rudd, J.D. Friesen 1992. Mutations causing aminotriazole resistance and temperature sensitivity reside in gyrB, which encodes the B subunit of DNA gyrase. J.Bacteriol. 174:5479-5481
- Lewis, R.J., O.M. Singh, C.V. Smith, T. Skarzynski, A. Maxwell, A.J. Wonacott, D.B. Wigley 1996. The nature of inhibition of DNA gyrase by the coumarins and the cyclothialidines revealed by X-ray crystallography. EMBO J. 15:1412-1420
- Kataoka, K., T. Mizushima, Y. Ogata, T. Miki, K. Sekimizu 1996. Heat shock-induced DNA relaxation in vitro by DNA gyrase of Escherichia coli in the presence of ATP. J.Biol.Chem. 271:24806-24810