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Site dut
- Name: dut
- Type: Gene
- Synonyms
b3640 MG1655 locus tag dnaS sof - Mnemonic: dUTPase
- Left End Point: 82.16
- Right End Point: 82.17
- Products:
deoxyuridinetriphosphatase dUTP nucleotidohydrolase dUTPPase dUTP pyrophosphatase - Direction: >
- Priority: 1
- 2 Alleles of This Gene
dut-1 dut-791-YFP(::cat) - External Database Links:
Host Site Page Links EcoGene.org EG10251 EcoliWiki dut - Comment:
- Comment on dut-1: TS thymidine auxotrophs; leaky dUTPase. Thy- due to a lack of dUMP for syntheesis of TMP by thymidilate synthetase. Thy+ at 25C
- References:
- Taylor, A.F., P.G. Siciliano, B. Weiss 1980. Cloning of the dut (deoxyuridine triphosphatase) gene of Escherichia coli. Gene 9:321-336
- Lundberg, L.G., H.O. Karlstrom, P.O. Nyman 1983. Isolation and characterization of the dut gene of Escherichia coli II. Restriction enzyme mapping and analysis of polypeptide products. Gene 22:127-131
- Lundberg, L.G., H.-O. Thoresson, H.O. Karlstrom, P.O. Nyman 1983. Nucleotide sequence of the structural gene for dUTPase of Escherichia coli K-12. EMBO J. 2:967-971
- Spitzer, E.D., B. Weiss 1985. The dfp gene of Escherichia coli K-12, a locus affecting DNA synthesis, codes for a flavoprotein. J.Bacteriol. 164:994-1003