E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Site cls

• Name: cls
• Type: Gene
  Synonyms

  b1249	MG1655 locus tag
  nov	Ivanisevic et al.

  
  
• Mnemonic: cardiolipin synthase
• Left End Point: 28.13
• Right End Point: 28.17
  Products:

  cardiolipin synthase

  
  
• Direction: <
• Priority: 1
  1 Alleles of This Gene

  cls-756(del)::kan

  
  
• External Database Links:

  Host Site	Page Links
  NCBI/GenBank	D38779
  NCBI/GenBank	U15986
  EcoGene.org	EG11608
  EcoliWiki	cls

  
  
• Comment:
  • Selectable phenotype: Dihydroxybutyl 1-phosphonate resistance. Mutants have incr. sens. to novobiocin and reduced levels of cardiolipin.
• References:
  • Hwang, Y.-W., R. Engel, B.E. Troop 1984. Correlation of 3,4-dihydroxybutyl 1-phosphonate resistance with a defect in cardiolipin synthesis in Escherichia coli. J.Bacteriol. 157:846-856
  
  
  • Ohta, A., T. Obara, Y. Asami, I. Shibuya 1985. Molecular cloning of the cls gene responsible for cardiolipin synthesis in Escherichia coli and phenotypic consequences of its amplification. J.Bacteriol. 163:506-514
  
  
  • Nishijima, M., Y. Asami, N. Uetake, S. Yamagoe, A. Ohta, I. Shibuya 1988. Disruption of the Escherichia coli cls gene responsible for cariolipin synthesis. J.Bacteriol. 170:775-780
  
  
  • Heber, S., B.E. Tropp 1991. Genetic regulation of cardiolipin synthase in Escherichia coli. Biochim.Biophys.Acta 1129:1-12
  
  
  • Hiraoka, S., K. Nukui, N. Uetake, A. Ohta, I. Shibuya 1991. Amplification and substantial purification of cardiolipin synthase of Escherichia coli. J.Biochem.(Tokyo) 110:443-449
  
  
  • Hiraoka, S., H. Matsuzaki, I. Shibuya 1993. Active increase in cardiolipin synthesis in the stationary growth phase and its physiological significance in Escherichia coli. FEBS Lett. 336:221-224
  
  
  • Ivanisevic, R., M. Milic, D. Ajdic, J. Rakonjac, D.J. Savic 1995. Nucleotide sequence, mutational analysis, transcriptional start site, and product analysis of nov, the gene which affects Escherichia coli K-12 resistance to the gyrase inhibitor novobio
  
  
  • Tropp, B.E., L. Ragolia, W. Xia, W. Dowhan, R. Milkman, K.E. Rudd, R. Ivanisevic, D.J. Savic 1995. Identity of the Escherichia coli cls and nov genes. J.Bacteriol. 177:5155-5157
  
  
  • Tropp, B.E. 1997. Cardiolipin synthase from Escherichia coli. Biochim.Biophys.Acta 1348:192-200