- CGSC#: 4289
- Strain Designation
Designation Source Preference KL738 Cappello M.C. 1 MAF1/JC1553 Maas W.K. 2
- Sex(Hfr,F+,F-,or F'): F'
- 14 Mutations:
Mutation Location Certainty Display leuB6(Am) 1.74 1 leuB6(Am) fhuA2::IS2 3.61 1 fhuA2::IS2 lacY1 7.79 1 lacY1 glnX44(AS) 14.99 1 glnX44(AS) gal-6 16.96 1 gal-6 LAM- 17.40 1 λ- hisG1 45.01 1 hisG1 recA1 60.80 1 recA1 argG6 71.48 1 argG6 rpsL104 74.84 1 rpsL104 malT1(LamR) 76.54 1 malT1(λR) xyl-7 80.34 1 xyl-7 mtlA2 81.26 1 mtlA2 metB1 88.94 1 metB1
- 1.This strain should be maintained on medium containing met, his, leu and lacking arg. It grows slowly on such medium. Watch out for faster-growing clones, as these may be segregants with partial
- 2.deletions of F140. The strain gives Mtl- and Xyl- phenotypes on MacConkey agar plates. This is because on this rich medium the partial segregants completely overgrow the clones which still
- 3.contain the F140 intact. The strain will grow on minimal media appropriately supplemented with mannitol or xylose as carbon and energy source.
- Episome/Plasmid: F140
Plasmid Markers/Mutations: mtl-0