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Strain DE418

• CGSC#: 12351
• Strain Designation

  Designation	Source	Preference
  DE418	Stahl F.	1
  DE418/pLM2	Stahl F.	2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 6 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	0	λ-
  thyA36	63.86	0	thyA36
  IN(rrnD-rrnE)1	73.74	0	IN(rrnD-rrnE)1
  rph-1	82.20	0	rph-1
  rpoB331(rifR)	90.08	0	rpoB331(rifR)
  hsdR19	98.74	0	hsdR19

  
  
• Comments:
  • It is believed that this plasmid is in a W3110 background, but that has not been positively established. It is documented to be a non-suppressing host so it should be sensitive to ampicillin and tetracycline.
• Episome/Plasmid: pLM2

  Plasmid Markers/Mutations:
  ampR(Am)
  kan
  tet(Am)
  kan

• References:
  • Mindich, L., C.J. Cohen, M. Weisburd 1976. Isolation of nonsense suppressor mutants of Pseudomonas. J.Bacteriol. 126:177-182
  • Seed, B 1983. Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo. Nucleic Acids Res. 11(8):2427-45.
  
  

  Printable Strain Report