Strain - BW25993/pKD46

Strain - BW25993/pKD46Strain BW25993/pKD46

Strain BW25993/pKD46

CGSC#: 14176
Strain Designation
Designation Source Preference
BW25993/pKD46 Wanner B.L. 1
Sex(Hfr,F+,F-,or F'): F-
5 Mutations:
Mutation Location Certainty Display
DE(araD-araB)567 1.40 1 Δ(araD-araB)567
LAM- 17.40 1 λ^-
rph-1 82.20 1 rph-1
DE(rhaD-rhaB)568 88.16 1 Δ(rhaD-rhaB)568
hsdR514 98.74 1 hsdR514
Comments:
Episome/Plasmid: pKD46
Plasmid Markers/Mutations:
araBp-gam-bet-exo
bla(ApR)
oriR101
repA101(ts)
araC
bla
[tL3]
References:
- Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
Printable Strain Report

Mutation	Location	Certainty	Display
DE(araD-araB)567	1.40	1	Δ(araD-araB)567
LAM-	17.40	1	λ^-
rph-1	82.20	1	rph-1
DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
hsdR514	98.74	1	hsdR514