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Strain VIP205
- CGSC#: 7497
- Strain Designation
Designation Source Preference VIP205 Vicente M. 1 - Sex(Hfr,F+,F-,or F'): F-
- 14 Mutations:
Mutation Location Certainty Display DE(araA-leu)7697 1.35 1 Δ(araA-leu)7697 araD139([B/r]) 1.42 1 [araD139]B/r Omega kan,T,lacIQ 2.26 1 Ωkan,T,lacIQ P\_tac-ftsZ20 2.27 1 P\_tac-ftsZ20 DE(codB-lacI)3 7.60 1 Δ(codB-lacI)3 galK16 16.99 1 galK16 galE15(GalS) 17.03 1 galE15(GalS) LAM- 17.40 1 λ- e14- 26.00 1 e14- mcrA0 26.07 1 mcrA0 relA1 62.71 1 relA1 rpsL150(strR) 74.84 1 rpsL150(strR) mcrB9999 98.63 1 mcrB9999 hsdR2 98.74 1 hsdR2 - Comments:
- Because of the insertion cassette between ftsA and ftsZ, this strain requires IPTG for growth. See reference below, p. 3961
- In Lyophil Box #3
- References:
- Garrido, T., M. Sanchez, P. Palacios, M. Aldea, M. Vicente 1993. Transcription of ftsZ oscillates during the cell cycle of Escherichia coli. EMBO J. 12:3957-3965
- Palacios, P., M. Vicente, M. Sanchez 1996. Dependency of Escherichia coli cell-division size, and independency of nucleoid segregation on the mode and level of ftsZ expression. Mol.Microbiol. 20:1093-1098
- Flardh, K., T. Garrido, M. Vicente 1997. Contribution of individual promoters in the ddlB-ftsZ region to the transcription of the essential cell-division gene ftsZ in Escherichia coli. Mol.Microbiol. 24:927-936
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