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Strain DF225

• CGSC#: 5585
• Strain Designation

  Designation	Source	Preference
  DF225	Fraenkel D.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 15 Mutations:

  Mutation	Location	Certainty	Display
  leuB6(Am)	1.74	1	leuB6(Am)
  fhuA2::IS2	3.61	1	fhuA2::IS2
  lacY1	7.79	1	lacY1
  tsx-1	9.28	1	tsx-1
  glnX44(AS)	14.99	1	glnX44(AS)
  gal-6	16.96	1	gal-6
  LAM-	17.40	1	λ-
  gapA3	40.09	1	gapA3
  hisG4(Oc)	45.01	1	hisG4(Oc)
  argG6	71.48	1	argG6
  rpsL104	74.84	1	rpsL104
  malT1(LamR)	76.54	1	malT1(λR)
  xyl-7	80.34	1	xyl-7
  mtlA2	81.26	1	mtlA2
  metB1	88.94	1	metB1

  
  
• Comments:
  • The gap-3 marker reverts at a relatively high rate.
• References:
  • Hillman, JD, DG Fraenkel 1975. Glyceraldehyde 3-phosphate dehydrogenase mutants of Escherichia coli. J. Bacteriol. 122(3):1175-9.
  
  

  Printable Strain Report