Strain ts9
- CGSC#: 5593
- Strain Designation
Designation Source Preference ts9 Flaks J. 1 - Sex(Hfr,F+,F-,or F'): F-
- 16 Mutations:
Mutation Location Certainty Display leuB6(Am) 1.74 1 leuB6(Am) fhuA2::IS2 3.61 1 fhuA2::IS2 lacY1 7.79 1 lacY1 tsx-1 9.28 1 tsx-1 glnX44(AS) 14.99 1 glnX44(AS) gal-6 16.96 1 gal-6 LAM- 17.40 1 λ- hisG1 45.01 1 hisG1 argG6 71.48 1 argG6 malT1(LamR) 76.54 1 malT1(λR) xyl-7 80.34 1 xyl-7 mtlA2 81.26 1 mtlA2 metB1 88.94 1 metB1 coaA1(ts) 89.92 1 coaA1(ts) rplL9(L?) 90.06 1 rplL9(L?) ilu-1(ts) 1000.00 1 ilu-1(ts) - Comments:
- The mutation coaA1, originally called rts-1, confers temperature-sensitivity on this strain. The mutation ilu-1 confers a temperature-sensitive requirement for isoleucine.
- References:
- Flaks, J., P.S. Leboy, E.A. Birge, C.G. Kurland 1966. Mutations and genetics concerned with the ribosomes. Cold Spr.Harb.Symp.Quant.Biol. 31:623-631
- Watson, RJ, J Parker, NP Fiil, JG Flaks, JD Friesen 1975. New chromosomal location for structural genes of ribosomal proteins. Proc. Natl. Acad. Sci. U.S.A. 72(7):2765-9.
- Song, W.J., S. Jackowski 1992. Cloning, sequencing, and expression of the pantothenate kinase (coaA) gene of Escherichia coli. J.Bacteriol. 174:6411-6417
- Song, W.-J., S. Jackowski 1992. coaA and rts are allelic and located at kilobase 3532 on the Escherichia coli physical map. J.Bacteriol. 174:1705-1706
- Siri, M., S.K. Westaway, S.J. Hayflick 2004. Pers.Commun. 6-:2004
Printable Strain Report