Strain pLC34-20B
- CGSC#: 14150
- Strain Designation
Designation Source Preference pLC34-20B 1 - Sex(Hfr,F+,F-,or F'): F+
- 13 Mutations:
Mutation Location Certainty Display thr-1 0.01 1 thr-1 araC14 1.52 1 araC14 leuB6(Am) 1.74 1 leuB6(Am) lacY1 7.79 1 lacY1 glnX44(AS) 14.99 1 glnX44(AS) galK2(Oc) 16.99 1 galK2(Oc) galT22 17.01 1 galT22 LAM- 17.40 1 λ- trpE63(del) 28.44 1 ΔtrpE63 recA56 60.80 1 recA56 xylA5 80.34 1 xylA5 mtl-1 81.26 1 mtl-1 thiE1 90.34 1 thiE1 - Comments:
- Episome/Plasmid: pLC34-20B
Plasmid Markers/Mutations: araC14 galK2(Oc) galT22 glnX44(AS) lacY1 LAM- leuB6(Am) mtl-1 recA56 thiE1 thr-1 trpE63(del) xylA5 pyrH tdi-?? dnaE proS metD fadE rrnH rrsH alaV ileV rrlH rrfH aspU dnaQ rnhA strC - References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Clarke, L., J. Carbon 1979. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 68:396-408
- Nishijima, M., C.E. Bulawa, C.H.R. Raetz 1981. Two interacting mutations causing temperature-sensitive phosphatidylglycerol synthesis in Escherichia coli membranes. J.Bacteriol. 145:113-131
- Horiuchi, T., H. Maki, M. Maruyana, M. Sekiguchi 1981. Identification of the dnaQ gene product and location of the structural gene for RNase H of Escherichia coli by cloning of the genes. Proc.Natl.Acad.Sci.USA 78:3770-3774
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
Printable Strain Report