E. coli Genetic Resources at Yale
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Strain DF1020

• CGSC#: 6194
• Strain Designation

  Designation	Source	Preference
  DF1020	Fraenkel D.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 9 Mutations:

  Mutation	Location	Certainty	Display
  pro-82	5.90	1	pro-82
  glnV44(AS)	14.99	1	glnV44(AS)
  LAM-	17.40	1	λ-
  pfkB201(del)	38.89	1	ΔpfkB201
  recA56	60.80	1	recA56
  endA1	66.57	1	endA1
  DE(rhaD-pfkA)200	88.18	1	Δ(rhaD-pfkA)200
  thiE1	90.34	1	thiE1
  hsdR17	98.74	1	hsdR17

  
  
• Comments:
  • Reference below is to related strains DF1010. Derivation of DF1020 follows the same steps as DF1010, but the starting strain was MM294A rather than K10.
  • There are problems verifying the pfk- phenotype of this strain. Suggest using RL257 instead.
• References:
  • Daldal, F. 1983. Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme. J.Mol.Biol. 168:285-305
  
  

  Printable Strain Report