E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection
• The Database
• Privacy Policy
• Acknowledgment of Support

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Contact Information
• Charges
• Reviving Strains
• Strains Not Found?

Other CGSC Information

• FAQ on Procedures
• Current Working Map
• 1998 MMBR Map
• 1998 MMBR Gene List
• Map Diagrams (PDFs)

Other Links

• E. coli Links
• Other Stock Collections
• Other Bio Links
• Yale Web

Funded by a grant from the National Science Foundation

Strain BW20338

• CGSC#: 7917
• Strain Designation

  Designation	Source	Preference
  BW20338	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 7 Mutations:

  Mutation	Location	Certainty	Display
  DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
  phoA532(del)	8.64	1	ΔphoA532
  uidA4(del)::pir-116	36.48	1	ΔuidA4::pir-116
  recA1	60.80	1	recA1
  rpoS396(Am)	61.75	0	rpoS396(Am)
  rph-1	82.20	1	rph-1
  DE(phnP-phnD)3330( phnC?)	92.93	1	Δ(phnP-phnD)3330( phnC?)

  
  
• Comments:
• Episome/Plasmid: F128-Tn10-12

  Plasmid Markers/Mutations:
  F::Tn10-12(kan)
  lacIp-4000(lacIQ)
  lacZ58(del)(M15)
  proA
  proB

• References:
  • Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
  
  

  Printable Strain Report