- CGSC#: 4389
- Strain Designation
Designation Source Preference KL304 Cappello M.C. 1 KL303 3
- Sex(Hfr,F+,F-,or F'): F-
- 12 Mutations:
Mutation Location Certainty Display DE(gpt-proA)62 5.51 1 Δ(gpt-proA)62 lacY1 7.79 1 lacY1 glnX44(AS) 14.99 1 glnX44(AS) glnS1(ts) 15.20 1 glnS1(ts) galK2(Oc) 16.99 1 galK2(Oc) LAM- 17.40 1 λ- purB15 25.65 1 purB15 hisG4(Oc) 45.01 1 hisG4(Oc) rpsL35(strR) 74.84 0 rpsL35(strR) xylA5 80.34 1 xylA5 mtl-1 81.26 1 mtl-1 thiE1 90.34 1 thiE1
- glnS1 is a temperature-sensitive mutation. Growth stops immediately at 42 degrees C. This strain was made in the same way as was KL303 and is probably identical to KL303.
- hours of incubation. At 44 degrees C, the temperature sensitivity is more clear-cut. The glnS mutation reverts. The strain should be cloned and tested before use.
- This strain is temperature-sensitive at 42 degrees only on a medium containing a low salt concentration (e.g., Luria Agar modified to contain only 0.5% NaC1) and plates should be examined after 9
- Korner, A, BB Magee, B Liska, KB Low, EA Adelberg, D Soll 1974. Isolation and partial characterization of a temperature-sensitive Escherichia coli mutant with altered glutaminyl-transfer ribonucleic acid synthetase. J. Bacteriol. 120(1):154-8.