E. coli Genetic Resources at Yale
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Strain JT31

• CGSC#: 8194
• Strain Designation

  Designation	Source	Preference
  JT31	Wood J.M.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 7 Mutations:

  Mutation	Location	Certainty	Display
  lacZ1125	7.81	1	lacZ1125
  LAM-	17.40	1	λ-
  putA1::Tn5	23.15	1	putA1::Tn5
  trpA49(Am)	28.33	1	trpA49(Am)
  relA1	62.71	1	relA1
  rpsL150(strR)	74.84	1	rpsL150(strR)
  spoT1	82.34	1	spoT1

  
  
• Comments:
  • Cannot utilize proline as C or N source. More sensitive to L-azetidine-w-carboxylate and 3,4-dehydroproline than parent, CSH4
• References:
  • Wood, J.M. 1981. Genetics of L-proline utilization in Escherichia coli. J.Bacteriol. 146:895-901
  
  

  Printable Strain Report