Strain - RL254

Strain - RL254Strain RL254

Strain RL254

CGSC#: 8245
Strain Designation
Designation Source Preference
RL254 Lovingshimer M.R. 1
Sex(Hfr,F+,F-,or F'): F-

11 Mutations:

Mutation	Location	Certainty	Display
araD139([B/r])	1.42	1	[araD139]_B/r
lacIp-4000(lacIQ)	7.90	1	lacIp-4000(lacI^Q)
e14-	26.00	1	e14-
flhD5301	42.59	1	flhD5301
DE(fruK-yeiR)725(fruA25)	48.70	1	Δ(fruK-yeiR)725(fruA25)
relA1	62.71	1	relA1
rpsL150(strR)	74.84	1	rpsL150(strR)
rbsR22	84.84	1	rbsR22
pfkA203(del-ins)::FRT	88.49	1	pfkA203(del-ins)::FRT
DE(fimB-fimE)632(::IS1)	97.81	1	Δ(fimB-fimE)632(::IS1)
deoC1	99.48	1	deoC1

Comments:
References:
- Lovingshimer, M.R., D.A. Siegele, G.D. Reinhart 2006. Construction of an inducible, pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sources. Protein Expr.Purif. 46:475-482
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