L48      Source of Strain:
D.F. SilbertOther Designations:
F- Chromosomal Markers: araC14
, tfr-5Strain Comments:
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- fabD89(ts)-- is a temperature-sensitive mutation.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
- Harder, M.E., I.R. Beacham, J.E. Cronan, K. Beacham, J.L. Honegger, D.F. Silbert 1972. Temperature-sensitive mutants of Escherichia coli requiring saturated and unsaturated fatty acids for growth: isolation and properties. Proc.Natl.Acad.Sci.USA 69:310
- Semple, KS, DF Silbert 1975. Mapping of the fabD locus for fatty acid biosynthesis in Escherichia coli. J. Bacteriol. 121(3):1036-46.