CGSC Strain#:
Strain Designation: F155/KL250      Source of Strain: R.J. Kadner
Sex: F' Episome/Plasmid: F155
   Plasmid Markers/Mutations: att$\phi$80 , trp
Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnV44(AS), galK2(Oc), λ^-, trp-35, hisG4(Oc), gyrA12(NalR), recA1, rpsL31(strR), xylA5, mtl-1, argE3(Oc), thiE1, deoB13 or deoB14, thyA25 or thyA20
Strain Comments:

• This strain must be grown at 30 degrees C.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thyA25 or thyA20-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• NalR = Nalidix.acidResist.
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Press, R, N Glansdorff, P Miner, J De Vries, R Kadner, WK Maas 1971. Isolation of transducing particles of phi-80 bacteriophage that carry different regions of the Escherichia coli genome. Proc. Natl. Acad. Sci. U.S.A. 68(4):795-8.