CGSC Strain#: 6799
Strain Designation: BD2314     
Source of Strain: B. Duncan
Sex: F-
Chromosomal Markers: fhuA2::IS2,
lacY1,
tsx-70,
glnX44(AS)
,
gal-6,
λ-,
trpC45,
his-68,
purC50,
ung-152::Tn10,
tyrA2,
galP63,
Δ(gltB-gltF)500,
rpsL125(strR)
,
malT1(λ
R)
,
xyl-7,
mtlA2,
thiE1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- Δ(gltB-gltF)500-- :Glt- phenotype in gltB mutant requires presence of a gdhA mutation as well. 0.2% aspartate is a better N-source than glutamate because of uptake(Pahel et al).
- Δ(gltB-gltF)500 was formerly called aspB24
- Δ(gltB-gltF)500 was formerly called aspB21
- Δ(gltB-gltF)500 was formerly called gltB31
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- = tetracycline resistant
- ::IS2 = Insertion of IS2
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: - Duncan, BK 1985. Isolation of insertion, deletion, and nonsense mutations of the uracil-DNA glycosylase (ung) gene of Escherichia coli K-12. J. Bacteriol. 164(2):689-95.