CGSC Strain#: 6846
Strain Designation: PAT84     
Source of Strain: Ricard & Hirota
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
ftsZ844(ts)
,
fhuA2::IS2,
lacY1,
glnX44(AS)
,
λ-,
trp-1,
hisG1,
rfbC1,
thyA719,
malT1(λ
R)
,
xyl-7,
mtlA2,
ΔargH1,
rplL9(L?)
,
thiE1,
deo-73Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- thyA719-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- deo-73-- is either deoB or deoC.
- fhuA2::IS2 was formerly called fhuA2
- ftsZ844(ts) was formerly called ftsZ84
- ftsZ844(ts) was formerly called ftsA84
- ftsZ844(ts) was formerly called sulB84
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- L? = assignment uncertain
- LamR = Lambda-resistant
- ts = Temperature Sensitive
References: - Ricard, M., Y. Hirota 1973. Process of cellular division in Escherichia coli: physiology study on thermosensitive mutants defective in cell division. J.Bacteriol. 116:314-0
- Lutkenhaus, J., H.C. Wu 1980. Determination of transcriptional units and gene products from the ftsA region of Escherichia coli. J.Bacteriol. 143:1281-1288