CGSC Strain#: 6888
Strain Designation: AX655     
Source of Strain: J.R. Walker
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
ftsI2158(ts)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
qsr'-0,
glnX44(AS)
,
galK2(Oc)
,
λ-,
Rac-0,
hisG4(Oc)
,
rfbC1,
mgl-50,
rpsL31(strR)
,
kdgK51,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Δ(gpt-proA)62 was formerly called proA2
- ftsI2158(ts) was formerly called sep-2158
- ftsI2158(ts) was formerly called pbpB2158
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Fletcher, G, CA Irwin, JM Henson, C Fillingim, MM Malone, JR Walker 1978. Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-mur-ftsA-envA cluster as determined with specialized transducing lambda bacteriophages. J. Bacteriol. 133(1):91-100.