CGSC Strain#: 6938
Strain Designation: JS8      Source of Strain: Liebke & Speyer
Sex: F- Chromosomal Markers: araC14, leuB6(Am), secA206(aziR), fhuA23, ΔlacZ114(H114), proC83, tsx-67, purE42, glnX44(AS), galK2(Oc), λ^-, trpE38, his-208, argG77, rpsL109(strR), xylA5, mtl-1, ilvA681, rpoB342, thiE1, metA160, tsm
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal^- phenotype, sup-78,near his.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• tsm-- is a temperature-sensitive mutation which maps between ptsI and dapE.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• (H114) = lacZ deletion H114 from CSH16
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• aziR = azide resistance
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Liebke, HH, JF Speyer 1983. A new gene in E. coli RNA synthesis. Mol. Gen. Genet. 189(2):314-20.