CGSC Strain#: 4342
Strain Designation: EC-2     
Source of Strain: J.R. Beckwith
Sex: Hfr
Point of Origin#: 121(TP2)(ts)
Map Position: 71.01
Direction of Marker Transfer: Clockwise
Chromosomal Markers: Δ(gpt-lac)5,
glnX44(AS)
,
λ-,
relA1,
TP2,
thiE1Strain Comments: - Δ(gpt-lac)5-- is the deletion Δ( pro-lac)X111 of Jacob and originated in the strain Hfr 3000 X111.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- TP2-- is a transposition of FTS114- lac (F42-114) between rpsL and argG. argG < lacI-A rpsL
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
References: - Beckwith, J.R., E.R. Signer, W. Epstein 1966. Transposition of the lac region of E. coli. Cold Spr.Harb.Symp.Quant.Biol. 31:393-401
- Beckwith, J.R., E.R. Signer 1966. Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi 80. J.Mol.Biol. 19:254-265