CGSC Strain#: 4446
Strain Designation: LCB499      Source of Strain: Cruezet-Sigal
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA2::IS2, pro-33, lacY1, purE43, glnX44(AS), gal-6, λ^-, hisG1, malT1(λ^R), glgB2, xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• L? = assignment uncertain
• LamR = Lambda-resistant

References:

• Sigal, N., J. Puig 1968. Compt.Rend.Acad.Sci.Paris 267:1223-0
• Cattaneo, J, M Damotte, N Sigal, F Sanchez-Medina, J Puig 1969. Genetic studies of Escherichia coli K 12 mutants with alterations in glycogenesis and properties of an altered adenosine diphosphate glucose pyrophosphorylase. Biochem. Biophys. Res. Commun. 34(5):694-701.