CGSC Strain#: 4447
Strain Designation: LCB618      Source of Strain: Cruezet-Sigal
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA2, pro-33, lacY1, purE43, glnV44(AS), gal-6, λ^-, hns-1, hisG1(Fs), rfbC1, galP63, Δ(gltB-gltF)500, malT1(λ^R), glgC16, glgC3, xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• Δ(gltB-gltF)500-- :Glt^- phenotype in gltB mutant requires presence of a gdhA mutation as well. 0.2% aspartate is a better N-source than glutamate because of uptake(Pahel et al).
• glgC16-- : This strain may contain this mutation. P.C. to Barbara Bachmann from Dr. Sarwen Dhir.
• Δ(gltB-gltF)500 was formerly called gltB31
• Δ(gltB-gltF)500 was formerly called aspB21
• Δ(gltB-gltF)500 was formerly called aspB24
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• hns-1 was formerly called cur-1
• hns-1 was formerly called osmZ1
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Fs = Frameshift mutation
• L? = assignment uncertain
• LamR = Lambda-resistant

References:

• Sigal, N., J. Puig 1968. Compt.Rend.Acad.Sci.Paris 267:1223-0
• Cattaneo, J, M Damotte, N Sigal, F Sanchez-Medina, J Puig 1969. Genetic studies of Escherichia coli K 12 mutants with alterations in glycogenesis and properties of an altered adenosine diphosphate glucose pyrophosphorylase. Biochem. Biophys. Res. Commun. 34(5):694-701.