CGSC Strain#: 4488
Strain Designation: G240R4     
Source of Strain: A. Garen
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
phoA7(UGA)
,
phoA10,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
trpT152(UGAs)
,
rrnB-2,
mcrB1,
creC510Strain Comments: - phoA10-- does not affect the activity of the enzyme.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- trpT152(UGAs)-- suppresses phoA7
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- trpT152(UGAs) was formerly called sup-52
- trpT152(UGAs) was formerly called Su9+
- = missense
- Const = constitutive
- T2R = T2 Resistant
- UGA = opal(UGA) mutation
- UGAs = UGA suppressor
References: - Chan, T.S., A. Garen 1970. Amino acid substitutions resulting from suppression of nonsense mutations. V. Tyrptophan insertion by the Su9+ gene, a suppressor of the UGA nonsense triplet. J.Mol.Biol. 49:231-234