CGSC Strain#: 4499
Strain Designation: JC590     
Source of Strain: A.J. Clark
Sex: F'
Episome/Plasmid: F20
Plasmid Markers/Mutations: Chromosomal Markers: leuB6(Am)
,
fhuA2::IS2,
lacY1,
tsx-1,
glnX44(AS)
,
gal-6,
λ-,
hisG1,
argG6,
rpsL104,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1Strain Comments: - This strain must be grown on medium containing leu, his and lacking arg, met, thi. This F-prime is very unstable.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- xyl-7 was formerly called xylA7
- = strep-resistant
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: - Clark, A.J., W.K. Maas, B. Low 1969. Production of a merodiploid strain from a double male strain of E. coli K12. Mol.Gen.Genet. 105:1-15