CGSC Strain#: 4499
Strain Designation: JC590      Source of Strain: A.J. Clark
Sex: F' Episome/Plasmid: F20
   Plasmid Markers/Mutations:
Chromosomal Markers: leuB6(Am), fhuA2, lacY1, tsx-1, glnV44(AS), gal-6, λ^-, hisG1(Fs), argG6, rpsL104, malT1(λ^R), xyl-7, mtlA2, metB1
Strain Comments:

• This strain must be grown on medium containing leu, his and lacking arg, met, thi. This F-prime is very unstable.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• xyl-7 was formerly called xylA7
• = strep-resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Fs = Frameshift mutation
• LamR = Lambda-resistant

References:

• Clark, A.J., W.K. Maas, B. Low 1969. Production of a merodiploid strain from a double male strain of E. coli K12. Mol.Gen.Genet. 105:1-15