CGSC Strain#: 12118
Strain Designation: BW27749     
Source of Strain: B.L. Wanner
Sex: F-
Chromosomal Markers: Δ(araD-araB)567,
ΔlacZ4787(::rrnB-3)
,
λ-,
Δ(araH-araF)570(::FRT),
ΔaraEp-531::kan,
φPcp8araE535,
rph-1,
Δ(rhaD-rhaB)568,
hsdR514Strain Comments: - Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
- ΔlacZ4787(::rrnB-3)-- : 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
- ΔaraEp-531::kan-- has resistance marker useful for transfer of F-prime carrying Pcp- araE fusion.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- Δ(araD-araB)567 was formerly called Δ araBADAH33
- Δ(rhaD-rhaB)568 was formerly called Δ rhaBADLD78
- ΔlacZ4787(::rrnB-3) was formerly called ::rrnB-4
- ΔlacZ4787(::rrnB-3) was formerly called Δ lacZWJ16
- = kanamycin resistant
- ::FRT = Flp Recombinase Target
- ::rrnB-3 = 4 copies of rrnB inserted
References: - Khlebnikov, A., K.A. Datsenko, T. Skaug, B.L. Wanner, J.D. Keasling 2001. Homogeneous expression of the P(BAD) promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter. Microbiol 147:3241-3247