MI1      Source of Strain:
F- Chromosomal Markers: ileS1
, ΔargH1Strain Comments:
- We have not been able to trace the exact derivation of this strain. From what is known, it might be expected to carry a tsx marker and a tyrT marker, also.
- ileS1-- is an allele which results in a 300-fold higher apparent Km for Ile and can grow only when isoleucine is overproduced or added to the medium (and a 4-fold derepression of Ile synthesis enzymes).
- Iaccarino, M., P.E. Berg 1971. Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase. J.Bacteriol. 105:527-537