CGSC Strain#: 6678
Strain Designation: pLC3-13/JA200      Source of Strain:
Sex: F+ Episome/Plasmid:
   Plasmid Markers/Mutations: rpsT , dnaJ , lspA
Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnV44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1
Strain Comments:

• The Clarke and Carbon plasmid pLC3-13 carries dnaJ, rpsT, lspA and does ont carry ileS or dapB.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA56-- Amino acid substitution Arg 60 Cys
• galK2(Oc) was formerly called gal₂
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• thiE1 was formerly called thi-1
• ΔtrpE63 was formerly called DE(trpE5)
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Tokunaga, M., J.M. Loranger, H.C. Wu 1983. Isolation and characterization of an Escherichia coli clone overproducing prolipoprotein signal peptidase. J.Biol.Chem. 258:12102-12105
• Innis, M.A., M. Tokunaga, M.E. Williams, J.M. Loranger, S.-Y. Chang, S. Chang, H.C. Wu 1984. Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene. Proc.Natl.Acad.Sci.USA 81:3708-3712